目的 用大鼠Walker-256移植性肝癌模型研究癌痛消颗粒对大鼠肝癌miRNAs的调节作用,以探讨癌痛消颗粒抗肝癌的可能机制。方法 将60只大鼠移植性肝癌模型随机分为5组,即癌痛消颗粒高剂量组(A组)、中剂量组(B组)、低剂量组(C组)、5-Fu组(D组)、生理盐水组(E组),于造模后第8天对癌痛消治疗组及生理盐水组灌胃给药,5-Fu组腹腔注射给药,连续用药14 d后停药24 h,处死大鼠,取出瘤块,用实时定量聚合酶链反应(qRT-PCR)技术观察肝癌特定miRNAs(miR-199a-3p、miR-199a-5p、miR-18)表达水平。另设正常饲养组(F组)大鼠10只,不进行造模及给药,供实验结束时作空白对照用。结果 造模组与空白对照组比较miR-18有更高水平的表达,miR-199a-3p, miR-199a-5p表达水平降低;造模各组(A、B、C、D组)药物干预后与生理盐水组(E组)比较肝癌组织miR-18表达水平降低,miR-199a-3p, miR-199a-5p表达水平升高(P<0.01);和E组比较,A、B、C、D组均可明显调节miRNAs水平, 癌痛消中剂量组比5-FU组更为明显(P<0.01)。结论 口服癌痛消颗粒对大鼠移植性肝癌组织的miRNAs表达具有一定的调控作用,提示癌痛消的抗癌机制可能与调控肝细胞癌发生和发展相关的miRNAs水平有关。

To explore the effect of Aitongxiao granule(traditional Chinese medicines) therapy on miRNAs of replant liver cancer rats. METHODS The 60 replant liver cancer rats were randomly divided into high dose(A), middle dose(B), lower dose(C), 5-Fu(D),distilled water(E)groups. After 14 days of experimental treatment, the expressions of miR-199a-3p、miR-199a-5p 、miR-18 in liver cancer tissues were detected with qRT-PCR. RESULTS Before experimental treatment,in comparison with E group, the level of miR-18 was higher but level of miR-199a-3p, miR-199a-5p were lower in A、B、C、D groups(P<0.01). After 14 days of experimental treatment, in comparison with E group, the level of miR-18 was lower but level of miR-199a-3p, miR-199a-5p were higher in A、B、C、D groups(P<0.01).The more significant differences were found in B group than that in D group. CONCLUSION The Aitongxiao granule has the regulative effects on miRNAs of replant liver cancer rats . Its therapeutical effects relate to the miRNAs expressions of liver cellular cancer development .